Fluorescent Proteins, Volume 85, Second Edition (Methods in Cell Biology)
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Fluorescent Proteins, Volume 85, Second Edition (Methods in Cell Biology) |
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Fluorescent Proteins, Volume 85, Second Edition (Methods in Cell Biology) |
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基本信息·出版社：Academic Press
·页码：660 页
·出版日期：2007年12月
·ISBN：0123725585
·条形码：9780123725585
·装帧：精装
·正文语种：英语
·丛书名：Methods in Cell Biology
内容简介 在线阅读本书
This new edition of
Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross corrlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
编辑推荐 Review This relatively compact book is a summary of contemporary thinking about cancer immunotherapy, with a focus on the relationship between tumors and the immune response of the host. The book is edited by authorities on tumor biology and cancer immunotherapy and reflects the nascent synthesis of these disciplines. The chapters are written by highly respected investigators, and the topics span much of the breadth and depth of this evolving field. The book seems designed to provide an overview for those who wish to immerse themselves in this field and who have a working knowledge of immunotherapy but rudimentary familiarity with cancer immunobiology and therapy. - The New England Journal of Medicine, April 2008
Review This relatively compact book is a summary of contemporary thinking about cancer immunotherapy, with a focus on the relationship between tumors and the immune response of the host. The book is edited by authorities on tumor biology and cancer immunotherapy and reflects the nascent synthesis of these disciplines. The chapters are written by highly respected investigators, and the topics span much of the breadth and depth of this evolving field. The book seems designed to provide an overview for those who wish to immerse themselves in this field and who have a working knowledge of immunotherapy but rudimentary familiarity with cancer immunobiology and therapy. - The New England Journal of Medicine, April 2008
