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Methods of Biochemical Analysis, Green Fluorescent Protein: Properties, Applicat |
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Methods of Biochemical Analysis, Green Fluorescent Protein: Properties, Applicat |
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基本信息·出版社：John Wiley & Sons Inc
·页码：464 页
·出版日期：2005年10月
·ISBN：0471736821
·条形码：9780471736820
·装帧：精装
·正文语种：英语
·丛书名：Methods of Biochemical Analysis
·外文书名：绿色荧光蛋白: 特性、应用与协议
内容简介 在线阅读本书
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
作者简介 MARTIN CHALFIE, PhD, is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell.
STEVEN R. KAIN, PhD, manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years′ experience with a variety of biotechnology firms in the San Francisco Bay Area.
编辑推荐 Review
"...a magnificent text that will immensely benefit students and scientists from many biomedical areas." (Annals of Biomedical Engineering, August 2006)
"Any researcher, graduate student or educator with an interest in using GFP should read this book. It will become the ′bible for GFP′ users." (The Quarterly Review of Biology, June 2006)
Review
"...a magnificent text that will immensely benefit students and scientists from many biomedical areas." (Annals of Biomedical Engineering, August 2006)
"Any researcher, graduate student or educator with an interest in using GFP should read this book. It will become the ′bible for GFP′ users." (The Quarterly Review of Biology, June 2006)
